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pW60
pW60
規(guī)格:
貨期:
編號:B237390
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pW60
商品貨號 B237390
Designations pW60
Depositors T Lufkin
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.6700000762939450
DESCRIPTION OF VECTOR:
Intact vector size: 6.670
Type of vector: plasmid
Cloning sites: NotI, XbaI
Polylinker sites:
Construction: pBluescript KS+
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
other: internal ribosome entry sequence (IRES), ->
reporter group: lacZ, ->
replicon: pMB1
marker(s): ampR
Vector: pW60 (plasmid)
Construction: pBluescript KS+
Marker(s):ampR
Construct size (kb): 6.670000076293945
Features: marker(s): ampR
other: internal ribosome entry sequence (IRES)
replicon: pMB1
reporter group: lacZ
Applications
reporter construct beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 3.1; HindIII--6.4, 0.3.
Plasmid expressing beta-galactosidase. Foreign genes can be cloned upstream of the IRES/reporter gene, allowing production of a dicistronic messenger RNA in mammalian cells.
IRES/reporter insert can be released by XhoI.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Li X, et al. Dicistronic lacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes. BioTechniques 23: 874-882, 1997. PubMed: 9383553

Thomas Lufkin, personal communication

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