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in another host, produces protein CD40 antigen ligand

Construct size (kb): 3.859999895095825

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDL-SRalpha296
Intact vector size: 3.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Construction: pcDV1, HTLV-1 LTR
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40/HTLV-1 LTR
replicon: pMB1, SV40
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD40LG
Other gene symbols: CD40L
Gene name: CD40 antigen ligand
Contains complete coding sequence?: Y
Chromosome: X; Localization: X q26
Tissue: CD4-positive T-cells
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: L07414

Insert lengths(kb): 0.8999999761581421

Tissue: CD4-positive T-cells

Gene product: CD40 antigen ligand [CD40L]

Target Gene: CD40 antigen ligand

Restriction digests of the clone give the following sizes (kb): HindIII--3.4, 1.5; NcoI--3.5, 1.4; PvuII--4.9; SalI--2.4, 2.5; BamHI--3.9, 1.0.

The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.58; NcoI--0.78; PvuII--0.52.

Contains the complete coding sequence (nt 40-825 of the sequence record).

COS-7 cells transfected with this construct demonstrate good surface expression of the protein.

The vector is modified from pcD, with a promoter constructed from the SV40 early promoter and the R segment and U5 sequence of the long terminal repeat of human T-cell leukemia virus type 1 (HTLV-1 LTR).

Subcloned from pBS-hCD40L-6A9 (ATCC 79812/3). The 5' BamHI site is derived from the vector and the 3' end is the naturally occurring BamHI site (nt 929 of the published sequence).