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pCD2
pCD2
規(guī)格:
貨期:
編號(hào):B232183
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
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DNA
RNA

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凍干粉
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甘油
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產(chǎn)品名稱 pCD2
商品貨號(hào) B232183
Designations pCD2
GenBank Number

X63656

Species Escherichia coli (Migula) Castellani and Chalmers
Depositors The United States of America, RM Blaese, CA Mullen, The United States of America
Applications
in suitable host, produces protein cytosine deaminase
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: cytosine deaminase [codA]
Target Gene: cytosine deaminase
Insert Size (kb) 1.700
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4; BamHI--7.4; EcoRI/BamHI--5.7, 1.7.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.09; ClaI--0.67, 1.28; AccI--0.89.
Murine fibroblast cell lines such as NIH 3T3 and PA317, transformed with pCD2, express cytosine deaminase.
If transformed cells are grown in the presence of non-toxic 5-flurocytosine (5FC), the cytosine deaminase will convert the 5FC into the lethal metabolite 5-fluoruracil which inhibits DNA and RNA synthesis.
This cytosine deaminase negative selection system (CDNSS) could prove useful in selectively controlling gene therapy experiments.
PA317/pCD2 transformed cell lines expressed 16.3U of cytosine deaminase while NIH 3T3/pCD2 transformed cell lines expressed 3.6U (1U = 1pmol of uracil converted from cytosine/10+E08 cells/minute).
Recombinant cytosine deaminase is expressed poorly in eukaryotic cells if 88 bp 5' to naturally occurring GTG start site is present. pCD2 was engineered to remove these 88 bp and convert the GTG start to ATG.
Classification Enterobacteriaceae, Escherichia
References

Mullen CA, Blaese RM. Cytosine deaminase negative selection system for gene transfer techniques and therapies. US Patent 5,358,866 dated Oct 25 1994

Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. BioTechniques 7: 980-990, 1989. PubMed: 2631796

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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