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Rotavirus reassortant
Rotavirus reassortant
規(guī)格:
貨期:
編號:B229230
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Rotavirus reassortant
商品貨號 B229230
Classification Reoviridae, Rotavirus
Agent Rotavirus reassortant
Strain WI79(4+9) [WI79-4,9]
Applications
vaccine production
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Reassortant WI79-4,9, which contains gene segments 4 and 9 from the human rotavirus strain WI79, reassortants WI79-3,9 and WI79-4 were combined in a culture and treated with anti-bovine rotavirus serum.
WI79-4,9 was then identified among the progeny and harvested from the culture.
Product Format frozen
Storage Conditions -70°C
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
-70C
Propagate in cells fed with serum-free medium containing 0.5 ug/ml purified trypsin.
Effect on Host
Yes, in vitro effects: Cytopathic effects (cell clumping followed by cell death and lysis)
CPE is clumping followed by cell death and lysis
Recommended Host
MA-104 cells (ATCC CRL-2378)

Host of Choice: MA-104 (CRL-2378)

Host Range: MA-104, CV-1, Vero

MA-104 cells (ATCC CRL-2378); CV-1 cells (ATCC CCL-70); Vero cells (ATCC CCL-81)
Growth Conditions
Temperature: 37.0°C
Duration: 2-5 days at 37C
Cryopreservation
Storage temperature: -70C
Name of Depositor Wistar Institute of Anatomy and Biology
U.S. Patent
U.S. Patent Number
Special Collection NCRR Contract
Source
Reassortant WI79-4,9, which contains gene segments 4 and 9 from the human rotavirus strain WI79, reassortants WI79-3,9 and WI79-4 were combined in a culture and treated with anti-bovine rotavirus serum.
WI79-4,9 was then identified among the progeny and harvested from the culture.
References

Clark HF, et al. Rotavirus reassortant vaccine. US Patent 5,626,851 dated May 6 1997

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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