久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pdeltaADE2
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pdeltaADE2
pdeltaADE2
規(guī)格:
貨期:
編號(hào):B225804
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pdeltaADE2
商品貨號(hào) B225804
Designations pdeltaADE2
Depositors JD Boeke
Biosafety Level 1
Host
Escherichia coli MC1066
Vector Information
Size (kb): 8.4
DESCRIPTION OF VECTOR:
Intact vector size: 8.400
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: pBluescript, URA3, hisG, ADE2 sequences
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
restriction site: BamHI
other: ADE2 flanking sequence
coding sequence: hisG, ->
marker(s): URA3, ->
coding sequence: hisG, ->
other: ADE2 flanking sequence
restriction site: BamHI
replicon: pMB1
marker(s): ampR
Vector: pdeltaADE2 (plasmid)
Construction: pBluescript, URA3, hisG, ADE2 sequences
Marker(s):URA3,ampR
Construct size (kb): 8.4
Features: marker(s): URA3
marker(s): ampR
other: ADE2 flanking sequence
replicon: pMB1
restriction site: BamHI
coding sequence: hisG
Applications
contains sequence ATP phosphoribosyltransferase
marker deletion vector phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
produces protein uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2, 3.2; EcoRI--5.0, 3.4; HindIII--7.0, 1.3.
The two step selection process requires a ura3 transformation host (this host can be created using pJL164 (ATCC 87471)). After transformation with the BamHI digested plasmid, URA3 integrants are selected on ura- plates.
The designer deletion strain is then recovered by selection on 5-FOA plates (loss of URA3 and ADE2 markers by a homologous recombination event between the two hisG repeats).
The deleted host retains the coding sequence for six C-terminal amino acids of ADE2.
E. coli containing this plasmid should be grown on media lacking pyrimidines to select for URA3-containing cells.
This deleter vector is used to create designer yeast strains with a non-revertable ade2 auxotrophic marker deletion.
The 5.2 kb BamHI insert contains two direct repeats of the Salmonella hisG gene flanking URA3 and about 700 bp of homology to sequences upstream and downstream of the ADE2 gene flanking the hisG-URA3-hisG sequence.
Media ATCC® Medium 2057: M9 salts with supplements
Growth Conditions
Temperature: 37°C
References

Aparicio OM, et al. Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66: 1279-1287, 1991. PubMed: 1913809

Alani E, et al. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545, 1987. PubMed: 3305158

Jef D Boeke, personal communication

Related Products
component of:ATCC 87472
Shipped frozen
Shipping Information Distributed: freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
汉源县| 衡山县| 台东市| 綦江县| 黄石市| 乌拉特后旗| 太白县| 彭泽县| 鹤山市| 东光县| 教育| 阿坝县| 仪陇县| 淮南市| 寻甸| 宜黄县| 新平| 梅州市| 桂林市| 阜阳市| 静乐县| 资溪县| 黄大仙区| 金溪县| 于田县| 淳化县| 黄冈市| 海伦市| 彰武县| 两当县| 保山市| 桑日县| 毕节市| 姜堰市| 涿鹿县| 酒泉市| 新泰市| 新竹县| 扬中市| 富民县| 县级市|